Description
General description
Exosomes are a form of extracellular vesicle (EV), which may contribute to malignant transformation and the metastasis of cancer. Intercellular communication via exosomes has therefore attracted significant interest in the cancer research and cell communication disciplines. To elucidate these interactions, labeling techniques based on fluorescent dyes have been optimized.
The BioTracker™ exosome protein labeling probe series can be used for staining the proteins of purified exosomes, which in turn permits imaging of labeled exosomes taken up by cells.SCT268, SCT269, and SCT270 are optimized for the labeling of exosomal proteins. Three different fluorescence colors (green, red, far red) enable application in multiplexing experiments, such as observation of exosome localization in organelles.Note: Green protein label product is difficult to see because it is present in a small mass and is a colorless foam. The exosome labeling probe products contain filtration tubes for the removal of dyes unreacted after fluorescence labeling, as well as an optimized protocol for labeling exosomes. Add 10 uL DMSO to tube of green, red, or far red exosome protein dye to create stock solution.SPECTRAL PROPERTIES:Ex 488 nm/Em 490-540 nm
Features and Benefits
This live cell probe can be used for staining of purified exosomal protein, which allows imaging in the green fluorescence range of labeled exosomes taken up by cells.
Preparation Note
Store BioTracker™ Green Exosome Protein Labeling Kit at 2 - 8 °C, protected from light.After resuspending in DMSO, store stock solution at -20 °C, protected from light.
Other Notes
SCT268 can be used for staining of purified exosomal protein, which allows imaging of labeled exosomes taken up by cells.Labeling capacity:Protein amount : 1-10μg/ sample, Particle count : 10 to 100 x 10^8/sample(ultracentrifugation-purified exosome preparation)
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
