Description
General description
The newly developed SNAP id 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on western blots. With this unique vacuum-driven system, the length of time required for immunodetection is greatly reduced. The classical western blotting time frame of 4- 24 hours is now accomplished in 30 minutes with no quality and signal compromisation. All immunodetection steps after protein transfer to a membrane (i.e., blocking, washing, and primary and secondary antibody incubations) can be performed with the SNAP id 2.0 system. SNAP id 2.0 Mini Blot Holder accepts up to 7.5 × 8.4 cm blots.
Application
SNAP id 2.0 Protein Detection System-Mini (7.5 x 8.4 cm) has been used in western blot analysis.
Features and Benefits
SNAP id 2.0 Protein Detection System Features:
Processing of up to four blots at a time on a vacuum base with two individually controlled sides
Comprises of three removable blot holding frame sizes: MultiBlot, Mini, and Midi, to accommodate different blot sizes
Disposable blot holders, sized for MultiBlot, Mini, and Midi frames
Blot spacer required with first-generation SNAP id system is now integrated into the new blot holder
Extended and off-line blot processing options: frames have lids and can be removed from the base for extended incubation (one hour to overnight), incubation in a shaker, or incubation at 4 °C
Stackable frames so multiple blots can be processed at the same time;30-minute immunodetection with uniform signal across the blot
Greater than 80% antibody recovery using the SNAP id 2.0 Antibody Collection Trays
Compatible with nitrocellulose and polyvinylidene fluoride (PVDF) membranes
Works with the most blocking buffers and visualization methodologies (e.g. chemiluminescence, fluorescence, or colorimetric)
Packaging
This system includes the SNAP id® 2.0 Base, two Mini Blot Holding Frames, two Antibody Collection Trays, vacuum tubing, Rolling Pad & Blot Roller, two Wetting Trays and a Quick Start Guide
Other Notes
Replaces: WBAVDBASE
The SNAP id® 2.0 Protein Detection System is intended for research use only. It is not for use in diagnostic procedures.
Legal Information
SNAP id is a registered trademark of Merck KGaA, Darmstadt, Germany
