Merck, SRP0112-50UG, HDAC-10 human

General description
Human HDAC10, amino acids 1-481, GenBank Accession No. NM_032019, with N-terminal GST tag, MW = 79 kDa, expressed in baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.