Merck, SRP0247-10UG, MAP3K14(NIK) Active human

General description
Human MAP3K14 (mitogen-activated protein kinase kinase kinase 14 ; NIK ; GenBank Accession No. NM_003954), (amino acids 318-end) with N-terminal GST tag, MW = 88 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.