Merck, SRP0293-10UG, Cathepsin V Active human

Name: Cathepsin V Active human
Brand: Merck
SKU: SRP0293-10UG

Size:10 μg, Cathepsin L2, Cathepsin U, recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

Catalog Number: SRP0293-10UG

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Category

Recombinant Enzymes

Pack Size10 µg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(amino acids 1-16) + cathepsin V (amino acids 18-334), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.
Application
Active human cathepsin V is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin V has been used in a study to assess the cysteine protease attribute of the eukaryotic ribosomal protein S4. Active human cathepsin V has also been used in a study to determine that the simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.
Physical form
Formulated in 40 mM MES, pH 5.0, 120 mM NaCL and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C
Legal Information
FreeStyle is a trademark of Invitrogen Corp.

Specifications

General

NACRES	NA.54
UNSPSC Code	12352204
recombinant	expressed in FreeStyle™ 293-F cells
assay	≥90% (SDS-PAGE)
form	aqueous solution
specific activity	≥436 pmol/min-μg
mol wt	37 kDa
NCBI accession no.	NM_001333.2
shipped in	dry ice
storage temp.	−70°C
Gene Information	human ... CTSL2(1515)

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Merck, SRP0293-10UG, Cathepsin V Active human

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