Merck, SRP2030-5UG, Sp1 GC-box binding protein human

Name: Sp1 GC-box binding protein human
Brand: Merck
SKU: SRP2030-5UG

Size:5 μg, recombinant, expressed in insect cells, ≥75% (SDS-PAGE)

Catalog Number: SRP2030-5UG

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Category

Insect Cell Expression Systems

Pack Size5 µg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Sp1 transcription factor has been used as a standard in electrophoretic mobility shift assay.
Biochem/physiol Actions
Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well-characterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Specifications

General

NACRES	NA.26
UNSPSC Code	12352202
biological source	human
recombinant	expressed in insect cells
assay	≥75% (SDS-PAGE)
form	frozen liquid
mol wt	~81.6 kDa
packaging	pkg of 5 μg
storage condition	avoid repeated freeze/thaw cycles
concentration	200 μg/mL
technique(s)	electrophoretic mobility shift assay: suitable
color	clear colorless
NCBI accession no.	NM_138473
UniProt accession no.	P08047
shipped in	dry ice
storage temp.	−70°C
Gene Information	human ... SP1(6667)

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Merck, SRP2030-5UG, Sp1 GC-box binding protein human

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General

Notice

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