Merck, SRP3014-10UG, BDNF human

Name: BDNF human
Brand: Merck
SKU: SRP3014-10UG

Size:10 μg, Brain Derived Neurotrophic Factor, Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

Catalog Number: SRP3014-10UG

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Category

Growth Factors & Cytokines

Pack Size10 µg

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Growth Factors & Cytokines

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions.
Biochem/physiol Actions
Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B). BDNF also plays an important role in vascular function and participates in angiogenesis. It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Analysis Note
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Other Notes
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Specifications

General

NACRES	NA.32
UNSPSC Code	12352202
biological source	human
recombinant	expressed in E. coli
assay	≥95% (SDS-PAGE)
form	lyophilized
mol wt	27.0 kDa
packaging	pkg of 10 μg
technique(s)	cell culture \| mammalian: suitable
impurities	≤1.00 EU/μg endotoxin, tested
UniProt accession no.	P23560
shipped in	wet ice
storage temp.	−20°C
Quality Level	200
Gene Information	human ... BDNF(627)

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Merck, SRP3014-10UG, BDNF human

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