Merck, ST1032-100UL, Anti-TRB3 (1-145) Rabbit pAb

General description
Rabbit polyclonal antibdy supplied as undiluted serum that has been adsorbed against GST to remove GST-reactive antibodies. Recognizes the ~45 kDa TRB3 protein.
Recognizes the ~45 kDa TRB3 protein in HepG2 cells.
TRB3 is a protein that is reported to disrupt insulin signaling by binding directly to Akt and blocking activation of the kinase.
This Anti-TRB3 (1-145) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of TRB3 (1-145).
Immunogen
Mouse
a recombinant protein consisting of amino acids 1-145 of mouse TRB3, fused to GST
Application
Immunoblotting (1:2500)
Immunocytochemistry (1:2500)
Immunoprecipitation (1:250)

Physical form
Undiluted serum.
Preparation Note
For long-term storage aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Analysis Note
Positive Control
HepG2 cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Du, K., et al. 2003. Science300, 1574.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)