Merck, ST1529-100UG, Anti-HTT Mouse mAb (3F1)

General description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.
Recognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
This Anti-HTT Mouse mAb (3F1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of HTT.
Immunogen
A recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
U-2 OS cells, HeLa NE cells, Human salivary gland tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)