Merck, ST1549-50UG, Anti-FASN Mouse mAb (3F2-1F3)

General description
Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the FASN protein.
Recognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue.
Immunogen
Full-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein
Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
293 cells, HeLa cells, Human breast carcinoma tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Kristiansen, G., et al. 2010. Br. J. Cancer102, 1736.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)