Merck, ST1622-100UG, Anti-LEPRE1 Mouse mAb (3C7)

General description
Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.
Immunogen
A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (6 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, Human esophagus tissue
Other Notes
Choi, JW, et al. 2009. PLoS Genet.5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)