Merck, ST1672-100UG, Anti-DPYD Mouse mAb (7D4)

General description
Anti-DPYD, mouse monoclonal, clone 7D4, recognizes the ~100 kDa DPYD protein in HeLa and HeLa NE cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~100 kDa DPYD protein.
Recognizes the ~100 kDa DPYD protein in HeLa and HeLa NE cells.
Immunogen
A recombinant polypeptide corresponding to amino acids of 1-111 human DPYD, expressed as a GST fusion protein
Application
ELISA (see comments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, HeLa NE cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)