Merck, ST1699-100UG, Anti-NDE1 Mouse mAb (2G11-1C11)

General description
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.
Recognizes the ~40-45 kDa NDE1 protein in HeLa cells.
This Anti-NDE1 Mouse mAb (2G11-1C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of NDE1.
Immunogen
Full-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
Application

ELISA (see coments)
Immunoblotting (1-4 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells
Other Notes
Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)