Merck, STRN250-1KT, Spectrum™ Plant Total RNA Kit

General description
Click here to view the kit protocol
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
Application
Spectrum™Plant Total RNA Kit has been used:
to extract RNA from the material ground in liquid nitrogen
to isolate total RNA from mutant and wt (wild type) plantlets
to extract total RNA from leaf samples
to extract RNA from all plant tissues

Features and Benefits
Yields up to 60 μg of pure concentrated RNA per prep
Efficient protocol allows for RNA purification in 30 min or less
Specially designed for research with difficult plant tissues

Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC