Merck, T8154-20ML, Trypan Blue solution

General description
For the same product in a convenient lyophilized (powder) format, please see T6146

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.
Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.
Application
Trypan Blue solution has been used:
in cell viability assay to count viable and dead cells
in rescue assay to count viable cells
in trypan blue exclusion counting method to determine proliferation curve
as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
to count GEC cells using a hemocytometer

Preparation Note
Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.