Merck, T9039-10PAK, Tris Buffered Saline, with Tween™ 20, pH 8.0

Application
Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay. It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.
Biochem/physiol Actions
Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer and also in blocking steps of immunoblotting technique.
Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C.
Legal Information
TWEEN is a registered trademark of Croda International PLC