Merck, TP0100-1KT, Total Protein Kit, Micro

General description
Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.
Application
Colorimetric, Endpoint
Suitable for protein determination for samples of up to 0.5 mg/ml.
Total protein kit, micro has been used to determine:
total protein in the bacterial homogenate by Bradford method
total protein content by the Bradford microassay from the tissue extracts
serum total protein
soluble protein content by the Bradford method from leaves

Biochem/physiol Actions
Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.
Total protein kit, micro can be used to determine the concentration of proteins in solution.