General description
The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.
CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.
CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.
The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:
A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes
THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT
[email protected].
Other Notes
1) 10ug CET 1019 HD-puro-SceI (+) Vector (CS221292)
2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))
3) 10ug DC HD-puro (-) Vector (CS221283)
4) 10ug DC AD-puro (-) Vector (CS221295)
Concentration: Please refer to lot specific datasheet.
Legal Information
UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany
