Merck, UFC510024, Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

Name: Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
Brand: Merck
SKU: UFC510024

Size:24 pkg, Centrifugal concentrator, 100 kDa MWCO, 0.5 mL sample volume, sample volume 0.5 mL, regenerated cellulose membrane, MWCO 100 kDa

Catalog Number: UFC510024

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Category

Ultrafiltration Spin Columns

TagsFiltration Concentration Filters Amicon<sup>®</sup> Centrifugals

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
Desalting, buffer exchange, and protein dialysis

Features and Benefits
Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
Concentrates 500 μL down to 15 μL
Concentration factor 25x to 30x
High sample recovery (greater than 90% of dilute starting solution)
Fast processing time: 10 to 30 minutes
Reverse spin capability provides consistent recoveries

Physical form
Color Code: Clear
Other Notes
Replaces: 42414
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

Diameter (Metric)	10.8 mm
Filtration Area	1 cm<sup>2</sup>
For Use With (Application)	Protein purification, protein concentration and desalting
Housing Material	Regenerated Cellulose (RC)
Includes	Two microcentrifuge tubes
Length (English)	0.196 in.
Length (Metric)	4.99 cm
Max. Force	14,000 xG for concentration spin; 1000 xG for reverse spin
Quantity	24Pack
Type	Centrifugal Filters
Volume (Metric)	0.5 mL
Disposable	Single-use
MWCO	100 kDa
Material (Membrane)	High-recovery Ultracel-PL Regenerated Cellulose
Sterility	Non-sterile
Unit Size	Each

FAQs

Question: What is the molecular-weight cutoff?

Answer:The membrane has a 100 kDa MWCO.

Question: What sample volume does the device accept?

Answer:The Ultra-0.5 format accepts up to 500 µL.

Question: How many filters are included?

Answer:This configuration contains 24 centrifugal filter devices.

Application Note

Amicon® Ultra-0.5 centrifugal filters are small-volume ultrafiltration devices for concentrating and buffer exchanging proteins and other macromolecules. This configuration uses a regenerated-cellulose membrane with a 100 kDa molecular-weight cutoff, accepts up to 0.5 mL of sample, and is supplied as 24 devices. The filtration area is approximately 1 cm², making the format suitable for low-volume research samples where recovery and concentration factor are more important than processing large feed volumes. Molecules above the nominal cutoff are retained while smaller solutes pass through the membrane during centrifugation. The device can also be used for desalting and buffer exchange through repeated dilution and concentration cycles. These filters are supplied non-sterile.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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