Merck, UFC910096, Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

Name: Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
Brand: Merck
SKU: UFC910096

Size:96 pkg, 100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator, sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa

Catalog Number: UFC910096

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Category

Ultrafiltration Spin Columns

TagsFiltration Concentration Filters Amicon<sup>®</sup> Centrifugals

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
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Application
Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
Concentration of dilute or pre-purified proteins from column eluents.
Desalting, buffer exchange and protein dialysis.

Features and Benefits
Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
Heat-sealed membrane minimizes downstream extractables.
100% integrity tested for reliable performance.
Convenient sample monitoring with translucent housing and volume gradations.
Direct pipettor sample access eliminates processing step to recover concentrate.
High concentration factors of 80–100X.

Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

Diameter (Metric)	29.7 mm
Filtration Area	7.6 cm<sup>2</sup>
For Use With (Application)	Protein purification, protein concentration and desalting
Housing Material	Regenerated Cellulose (RC)
Includes	Two microcentrifuge tubes
Length (Metric)	121 mm
Quantity	96Pack
Type	Centrifugal Filters
Volume (Metric)	15 mL
Disposable	Single-use
MWCO	100 kDa
Material (Membrane)	High-recovery Ultracel-PL Regenerated Cellulose
Sterility	Non-sterile
Unit Size	Each

FAQs

Question: What is the molecular-weight cutoff?

Answer:This configuration uses a 100 kDa MWCO membrane.

Question: What sample volume can be processed?

Answer:The device accepts up to 15 mL of sample.

Question: How many filters are supplied?

Answer:The package contains 96 centrifugal filter devices.

Application Note

Amicon® Ultra-15 centrifugal filters provide rapid concentration and buffer exchange of macromolecules using a regenerated-cellulose ultrafiltration membrane. This configuration has a 100 kDa molecular-weight cutoff, accepts up to 15 mL of sample, and is supplied as 96 devices. The membrane area is approximately 7.6 cm², supporting efficient processing of larger laboratory sample volumes by centrifugation. A dead-stop feature helps prevent the sample from spinning completely dry, while the vertical membrane design supports high recovery and convenient concentrate retrieval. The 100 kDa cutoff is useful for concentrating large proteins, protein complexes, nanoparticles, and other macromolecular species while smaller solutes pass through the membrane. The device can also be used for desalting and buffer exchange through repeated concentration and refill cycles. Select centrifugal force and spin time for the sample viscosity and target concentration.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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