Merck, UPBST-RT-HC-8KU, Ultra pure BST-RT Polymerase

General description
BST-RT Polymerase is an engineered Bst enzyme with robust reverse transcriptase (RT) activity, making it ideal for use in loop-mediated isothermal amplification (LAMP) or RT-LAMP assays. This feature eliminates the necessity of a secondary RT enzyme when targeting amplification from RNA samples, simplifying reaction setup. BST-RT works optimally at 65°C and can be used for real time reaction monitoring with a fluorescent dye or in endpoint detection assays. BST-RT polymerase is tolerant to potential reaction inhibitors such as ethanol, extraction buffers, and saliva. This enzyme is also compatible with workflows that incorporate dUTP for elimination of reaction carryover contaminants.
Application
Loop-mediated isothermal amplification (LAMP) has emerged asa key alternative to polymerase chain reaction (PCR)-based techniques foramplifying nucleic acids. While conventional PCR employs a thermal cycler forrepetitive cycling temperatures when amplifying, isothermal amplificationtechniques occur at a single and fixed temperature, allowing the use of simple,portable, and more robust instruments for fast and exponential amplification.
Features and Benefits

• Compatible with samples containing either DNA orRNA
• Ability to perform RT-LAMP without additional RTenzymes
• Lacks 5’ to 3’ exonuclease domain
• DNase free, RNase free, DNA free, and Nickasefree
• Active from 25-65 °C (optimal at 65 °C)
• Unit Definition: One unit is the amount ofenzyme required to incorporate 10 nmol of dNTP onto ssDNA template for 30minutes at 65°C.
• Also available in stand-alone, highconcentrations and glycerol-free formulations

Preparation Note
In solution.
Other Notes
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.Order primers for LAMP using our online configurator. For assistance with primer design prior to purchase, please use the online design tool from our partner.