Merck, WTA1-50RXN, TransPlex® Whole Transcriptome Amplification Kit

General description
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Application
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.
Suitable for use with downstream applications including:
qPCR
microarray analysis
cloning

Biochem/physiol Actions
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Features and Benefits
Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
Only 5 ng of starting material required to produce a highly representative library from total RNA
Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Legal Information
TransPlex is a registered trademark of Rubicon Genomics, Inc.