Description
General description
The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix™, especially formulated for amplification directly from extract.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR ReadyMix, included in the kit.
The Extract-N-Amp™ Plant PCR Kits are intended for direct PCR (polymerase chain reaction). This kit contains all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. It is a novel Extraction Solution that eliminates the need for conventional freezing of plant tissues.
The PCR reaction mix facilitates amplification directly from the extract by using an antibody-based hot-start DNA polymerase for specific amplification.
Application
Extract-N-Amp™ Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).
Extract-N-Amp™ Plant PCR Kit is suitable for use in genotyping.
It has been used forDNA extraction from fresh mushrooms.
Features and Benefits
Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
A PCR ReadyMix™, specially formulated for amplification directly from extract
Hot Start antibody for highly specific PCR amplification of genomic DNA
Extract stable at 4 °C for at least 6 months
Plant genomic DNA can be extracted for PCR in under 15 minutes using a method.
No freezing, mechanical disruption, organic extraction, column purification or precipitation is necessary
A specially formulated PCR ReadyMix is provided for use with the extracted DNA
The Hot Start antibody ensures highly specific PCR amplification of genomic DNA
Suitable for high-throughput plant genetic analysis.
Lengthy enzymatic digestions are not required, saving time and effort.
The extracted DNA remains stable at 4 °C for atleast 6 months.
Other Notes
The Extract-N-Amp Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
Extract-N-Amp™ Plant PCR Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix (2X) (buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody)
Extraction Solution- E7526-12 mL
Dilution Solution- D5688-12mL
Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml
Collection tubes
The Extract-N-Amp™ Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
