General description
The REDExtract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix™, especially formulated for amplification directly from extract. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the REDExtract-N-Amp ReadyMix, included in the kit.
The REDExtract-N-Amp™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Application
REDExtract-N-Amp™ Plant PCR Kit has been used to:
extract genomic DNA from mycelial lawns and leaves
screen leaf tissues from putative transgenic potato plants
perform polymerase chain reaction (PCR)
Features and Benefits
Genomic DNA for PCR in less than 15 minutes
A PCR ReadyMix™, specially formulated for amplification directly from extract
Hot Start antibody for highly specific PCR amplification of genomic DNA
Extract stable at 4 °C for at least 6 months
Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
No freezing, mechanical disruption, organic extraction, column purification or precipitation required
Specially formulated PCR ReadyMix for use with extract
Hot Start antibody for highly specific PCR amplification of genomic DNA
REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis
Compatible with high-throughput requirements for genetic analysis of plants
Extract stable at 4 °C for at least 6 months
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
