Merck, Y2377-250G, 2x YT medium

Name: 2x YT medium
Brand: Merck
SKU: Y2377-250G

Size:250 g, 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media, Powder microbial growth medium

Catalog Number: Y2377-250G

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Category

Dehydrated Culture Media

Pack Size250 g

TagsIndustrial Microbiology Microbial Culture Media

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.
Features and Benefits
2xYT powder provides:Standard formulation
An inexpensive alternative to liquid

Preparation Note
1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Specifications

General

NACRES	NA.85
UNSPSC Code	41106225
grade	Molecular Biology
sterility	non-sterile
form	powder
technique(s)	microbiological culture: suitable
pH	6.8-7.2(3.1% solution)
application(s)	food and beveragesmicrobiology
storage temp.	room temp
suitability	nonselective for Escherichia coli
Quality Level	200

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