Phenomenex, 00D-4601-E0, Kinetex 5 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Catalog Number: 00D-4601-E0
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| Pore Size | 100.0000 Å |
|---|---|
| Max Backpressure (bar) | 1034.0000 bar |
| Max Temperature (°C) | 60.0000 °C |
| Length (mm) | 100.0000 mm |
| Shipping Solvent | Acetonitrile /Water (50:50) |
| Particle Size | 5.0000 µm |
| Internal Diameter | 4.6000 mm |
| Film Thickness | 0.0000 µm |
Answer:The column measures 100 × 4.6 mm and uses 5 µm Kinetex C18 core-shell particles with 100 Å pores.
Answer:It is a reversed-phase C18 HPLC column used for hydrophobic separation of compatible analytes.
Answer:Yes. Phenomenex provides a chlorogenic acid separation example using an aqueous formic-acid/acetonitrile gradient; validate conditions for the intended sample.
The Phenomenex Kinetex C18 column is a reversed-phase liquid chromatography column with a 100 mm length and 4.6 mm internal diameter, packed with 5 µm core-shell particles and a nominal 100 Å pore size. Its C18 bonded phase separates compounds primarily through hydrophobic retention and is suitable for method development and routine reversed-phase analysis of compatible small molecules. The core-shell particle structure supports efficient mass transfer while permitting operation on conventional HPLC systems with suitable pressure capability. A published application demonstrates chlorogenic acid analysis using an acidified water–acetonitrile gradient, but separation conditions must be optimized for the actual sample and detector. Before use, equilibrate the column with a miscible mobile phase, filter or clarify samples, and monitor changes in backpressure and peak symmetry. Use only solvent compositions and operating conditions allowed by the column manual; a documented example is not a universal validated assay.
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