Phenomenex, 00H-2145-K0, BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea
Catalog Number: 00H-2145-K0
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| Length (mm) | 300.0000 mm |
|---|---|
| Particle Size | 5.0000 µm |
| Internal Diameter | 7.8000 mm |
| Film Thickness | 0.0000 µm |
Answer:The BioSep SEC-s2000 column uses 5 µm particles with 145 Å pores and measures 300 × 7.8 mm.
Answer:It uses size-exclusion, or gel-filtration, chromatography to differentiate molecules by hydrodynamic size.
Answer:The column has a nominal exclusion limit of approximately 300,000 Da; practical separation depends on analyte structure and mobile-phase conditions.
The Phenomenex BioSep SEC-s2000 is an analytical size-exclusion HPLC column with 5 µm particles, 145 Å nominal pore size and dimensions of 300 × 7.8 mm. It provides gel-filtration separation of proteins and peptides through differences in their hydrodynamic dimensions, making it useful for examining size variants, aggregation and low-molecular-weight components. The SEC-s2000 phase has a stated exclusion limit of approximately 300 kDa and supports isocratic methods developed around the target sample matrix. Buffer ionic strength, pH and organic modifiers can affect secondary interactions and peak shape; chromatography conditions should therefore be optimized for the analyte set. The 300 mm bed length supplies analytical separation path length, while the 7.8 mm internal diameter suits common SEC HPLC configurations. This is a single packed LC column, not bulk chromatography resin or a complete SEC instrument. Suitable guard-column selection and sample clarification help protect the packed bed.
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