Phenomenex, 00H-4788-K0, Biozen 3 µm dSEC-2, 200 Å, LC Column 300 x 7.8 mm, ea
Catalog Number: 00H-4788-K0
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| Length (mm) | 300.0000 mm |
|---|---|
| Particle Size | 3.0000 µm |
| Internal Diameter | 7.8000 mm |
| Film Thickness | 0.0000 µm |
Answer:3 µm packing, 200 Å pores, and a 300 × 7.8 mm analytical format.
Answer:Size-exclusion chromatography (SEC), separating analytes primarily by molecular size.
Answer:Applications include monoclonal-antibody aggregate, monomer and related biopharmaceutical size-variant analysis.
Phenomenex bioZen™ dSEC-2 is a size-exclusion chromatography column for characterization of therapeutic proteins and other biomolecules. This 300 × 7.8 mm analytical column contains 3 µm packing with 200 Å pores to separate species principally by hydrodynamic size, rather than by reversed-phase hydrophobic retention. It is relevant to measurements of monoclonal-antibody monomers, soluble high-molecular-weight aggregates and some lower-molecular-weight protein fragments within an appropriately qualified SEC method. The dSEC-2 stationary phase is designed to support good recovery and repeatable size-based separations with compatible aqueous mobile phases. Applications include biopharmaceutical stability evaluation, biosimilar comparability and protein quality control. Selection of buffer composition and detector conditions is important because nonspecific interactions can interfere with ideal size exclusion. This configuration is supplied as one analytical LC column.
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