Promega, A1222, PureYield™ Plasmid Miniprep System

Plasmid Minipreps in Minutes
The PureYield™ Plasmid Miniprep System isolates up to 15μg of transfection-quality plasmid DNA from 600μl to 3ml of bacterial culture in 10 minutes. The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the performance in applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.
The PureYield™ Plasmid Miniprep System can be used with centrifugation or vacuum-based protocols, and includes a convenient lysis/neutralization indicator dye.
This miniprep kit can be used to isolate any plasmid from E. coli hosts but works most efficiently when the plasmid is up to 20,000bp in size. For best results, use plasmids that are 10,000bp or less. Purified plasmid can be used without further manipulation for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. When used for in vitro transcription reactions, the isolated plasmid DNA should be supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).