Promega, A2492, PureYield™ Plasmid Midiprep System

Name: PureYield™ Plasmid Midiprep System
Brand: Promega
SKU: A2492

Up to 200µg of Transfection-Ready Plasmid DNA from 50ml Cultures, 30-minute, vacuum-based protocol, Highly pure, concentrated plasmid DNA, No tedious, gravity-drip columns or post-elution alcohol precipitation, Configuration: 25 preps

Catalog Number: A2492

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Category

Plasmid Purification Kits

TagsNucleic acid extraction Plasmid Purification

Description

The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.
PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.

Specifications

{"type": "table", "rows": [{"name": "Neutralization Solution (NSB)", "conditions": {"Part #": "A148H", "Size": "1 × 130ml", "Available Separately": "View Product"}}, {"name": "PureYield™ Binding Columns", "conditions": {"Part #": "A245A", "Size": "5 × 5 each", "Available Separately": ""}}, {"name": "PureYield™ Clearing Columns", "conditions": {"Part #": "A246A", "Size": "5 × 5 each", "Available Separately": ""}}, {"name": "Endotoxin Removal Wash", "conditions": {"Part #": "A251C", "Size": "1 × 85.3ml", "Available Separately": ""}}, {"name": "Column Wash", "conditions": {"Part #": "A252C", "Size": "1 × 210ml", "Available Separately": ""}}, {"name": "Cell Resuspension Solution (CRA)", "conditions": {"Part #": "A711F", "Size": "1 × 75ml", "Available Separately": "View Product"}}, {"name": "Cell Lysis Solution (CLA)", "conditions": {"Part #": "A712F", "Size": "1 × 75ml", "Available Separately": "View Product"}}, {"name": "Nuclease-Free Water", "conditions": {"Part #": "P119C", "Size": "1 × 25ml", "Available Separately": "View Product"}}]}

Ordering

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