Promega, A2495, PureYield™ Plasmid Midiprep System

Name: PureYield™ Plasmid Midiprep System
Brand: Promega
SKU: A2495

Up to 200µg of Transfection-Ready Plasmid DNA from 50ml Cultures, 30-minute, vacuum-based protocol, Highly pure, concentrated plasmid DNA, No tedious, gravity-drip columns or post-elution alcohol precipitation, Configuration: 100 preps

Catalog Number: A2495

Pricing

Send an RFQ.

View RFQ List

Category

Plasmid Purification Kits

TagsNucleic acid extraction Plasmid Purification

Description

The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.
PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.

Specifications

{"type": "table", "rows": [{"name": "Neutralization Solution (NSB)", "conditions": {"Part #": "A1485", "Size": "1 × 500ml", "Available Separately": ""}}, {"name": "PureYield™ Binding Columns", "conditions": {"Part #": "A245A", "Size": "20 × 5 each", "Available Separately": ""}}, {"name": "PureYield™ Clearing Columns", "conditions": {"Part #": "A246A", "Size": "20 × 5 each", "Available Separately": ""}}, {"name": "Endotoxin Removal Wash", "conditions": {"Part #": "A251B", "Size": "1 × 315ml", "Available Separately": ""}}, {"name": "Column Wash", "conditions": {"Part #": "A252B", "Size": "2 × 381ml", "Available Separately": ""}}, {"name": "Cell Resuspension Solution (CRA)", "conditions": {"Part #": "A7115", "Size": "1 × 315ml", "Available Separately": ""}}, {"name": "Cell Lysis Solution (CLA)", "conditions": {"Part #": "A7125", "Size": "1 × 315ml", "Available Separately": ""}}, {"name": "Nuclease-Free Water", "conditions": {"Part #": "P119C", "Size": "4 × 25ml", "Available Separately": "View Product"}}]}

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.