Description
Choose qPCR or RT-qPCR Master Mix Formats
Sensitivity and Specificity for qPCR
The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.
Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:
Sensitive detection for earlier quantitation of low- and high-copy targets.
Room-temperature setup,making it suitable for automation and high-throughput detection.
Compatibility with both fast and standard qPCR cycling methods.
Reverse Transcription and qPCR in One Reaction
The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.
Other features provided by the 1-Step System include:
Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
Two-Step Reverse Transcription and qPCR
The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.
The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl 2 , dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
