Description
High-Quality DNA and RNA for Advanced Downstream Analysis Assays
The Maxwell® HT XtractAll FFPE DNA/RNA Kit delivers high-quality DNA and RNA from a variety of tissue types. The nucleic acids extracted using this kit demonstrate excellent integrity and high yields of DNA and RNA, especially for longer fragment lengths as compared to other FFPE extraction offerings.
Comparison of DNA and RNA yields from a variety of FFPE tissue samples. DNA and RNA were extracted from multiple types of FFPE tissue samples (breast, liver, colon, kidney, uterine, endometrial, lung, cervical) using the Maxwell® XtractAll FFPE DNA/RNA chemistry sequential DNA/RNA workflow and two other commercially available sequential FFPE DNA/RNA extraction kits. DNA yield was evaluated by amplification using the ProNex® DNA QC Assay (Cat.# NG1002) for short (75bp; Panel A ) and long (300bp; Panel B ) target amplicons. RNA was evaluated by amplification assay for short (100bp; Panel C ) and long (171bp; Panel D ) target amplicons. The Maxwell® XtractAll FFPE DNA/RNA chemistry shows equivalent or superior performance for both DNA and RNA yields when evaluated by amplification assay, especially for longer target amplicons. Data represent mean ±SD, N=6.
Comparison of FFPE DNA and RNA fragment size and quality from several extraction kits. DNA and RNA were extracted from breast FFPE tissue samples using the Maxwell® XtractAll FFPE DNA/RNA chemistry sequential DNA/RNA workflow and two other commercially available sequential FFPE DNA/RNA extraction kits. Panel A. DNA fragment size was evaluated using the Agilent TapeStation D5000 ScreenTape Assay. Panel B. RNA fragment size was evaluated using the Agilent TapeStation RNA ScreenTape Assay, and DV200 values are shown. N=6.
This level of quality and consistency makes the Maxwell® HT XtractAll FFPE DNA/RNA Kit an ideal choice for demanding applications and sensitive assays, such as digital PCR or NGS.
Performance of Maxwell® XtractAll FFPE DNA/RNA in digital PCR. RNA was extracted from lung tumor FFPE samples using the Maxwell® XtractAll FFPE DNA/RNA chemistry and tested in EML4-ALK mRNA Fusions Assay (Bio-Rad). A 2D amplitude plot is shown; successful detection of the positive gene fusions is identified.
