Description
Choose qPCR or RT-qPCR Master Mix Formats
Sensitivity and Specificity for qPCR
The GoTaq® Probe qPCR Master Mix is a r eady-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. O ptimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl 2 , dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoTaq® Probe qPCR Master Mix offers:
Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
Optimized formulation compatible with running multiplex assays.
Room-temperature setup, making it suitable for automation and high-throughput detection.
Resistance to a wide range of PCR inhibitors.
qPCR amplification from FFPE samples with GoTaq ® P robe Master Mix . DNA (10ng) from a lung tumor FFPE sample was amplified under both standard and Fast thermal cycling conditions on a Roche LightCycler 480 instrument using a 384-well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.
Reverse Transcription and qPCR in One Reaction
The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
Expression levels for the Zmm19 gene in corn. Expression was evaluated via 1-step RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System (Cat.# A6120).
Two-Step Reverse Transcription and qPCR
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.
The GoTaq® Probe qPCR Master Mix contains GoTaq® Hot Start Polymerase, MgCl 2 , dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
Amplification curve for GAPDH detection from human pancreas RNA using the GoTaq® Probe 2-Step RT-qPCR System.
