Description
Choose qPCR or RT-qPCR Master Mix Format
GoTaq® Endure qPCR Master Mix
GoTaq® Endure qPCR Master Mix enables detection and relative quantification of DNA from challenging samples. GoTaq® Endure qPCR Master Mix is an all-in-one stabilized 2X hot-start formulation for qPCR. The master mix does not contain a reference dye; included is a separate tube of CXR Reference Dye, which has the same properties as ROX.
Inhibitor Tolerance of GoTaq® Endure qPCR Master Mix. Inhibitors were individually spiked at high concentrations to determine their effect on C q values. Shown is the maximum amount of each inhibitor that produced less than a 2C q shift of a 75bp target.
*Maximum amount tested; no inhibition observed.
GoTaq® Endure RT-qPCR System
The GoTaq® Endure RT-qPCR System enables detection and relative quantification of RNA expression levels from challenging samples using a one-step RT-qPCR method. The system combines GoScript™ Reverse Transcriptase and GoTaq® Endure qPCR Master Mix in a single-step real-time amplification reaction. The master mix does not contain a reference dye; included is a separate tube of CXR Reference Dye, which has the same properties as ROX.
GoScript™ Reverse Transcriptase utilizes the M-MLV Reverse Transcriptase enzyme and state-of-the-art buffer to drive robust, reliable cDNA synthesis with difficult templates in the presence of PCR inhibitors.
Inhibitor Tolerance of GoTaq® Endure RT-qPCR System. Inhibitors were individually spiked into purified RNA samples at high concentrations to determine their effect on C q values. Shown is the maximum amount of each inhibitor that produced less than a 2C q shift of a 75bp target.
