Promega, A6372, Maxwell® HT DNA FFPE Isolation System

FFPE System Performance Comparison
To demonstrate the performance of this system, Maxwell ® HT DNA FFPE purification chemistry was automated on the Tecan Freedom EVO ® and ThermoFisher KingFisher™ Flex instruments. Qiagen QIAamp ® and GeneRead kits were run using a standard method on a QIAcube instrument. Consecutive FFPE sections of human lung tumor and normal adjacent tissue (NAT) were interspersed among all methods and replicates to minimize sample-based variability. Eluates were quantitated using fluorometric detection and quality was assessed using the ProNex ® DNA QC Assay.
Next-Generation Sequencing Performance of DNA Purified with Maxwell® HT Chemistry. DNA purified using Maxwell® HT chemistry on both Tecan and KingFisher instruments was sequenced using an Illumina MiSeq after enrichment using the Ion AmpliSeq™ Cancer Hotspot Panel v2. Coverage, uniformity and depth were extremely high for both methods and for both tissue types, showing the suitability of these eluates in NGS applications. Furthermore, subsequent variant analysis revealed that low-frequency variants in the KRAS and EGFR genes were detected in the tumor tissue samples, but not in the normal tissue samples.