Promega, A8882, Direct PCR: XpressAmp® Direct Amplification Reagents

Viral RNA Detection Without the Need for Extraction
XpressAmp® Detection of RNA from Inactivated Influenza/RSV Virus Pellet
Amplification of Synthetic SARS-CoV-2 RNA from XpressAmp® Lysates
Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 10 4 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp® Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp® Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.