Promega, AM2030, GoTaq® Enviro qPCR and RT-qPCR Systems

Sensitive Detection for Environmental Samples
The GoTaq® Enviro qPCR and RT-qPCR Systems are ready-to-use master mixes optimized for amplifying targets from environmental samples (e.g., water, soil, biological material). The systems are resistant to a wide range of PCR and RT-qPCR inhibitors such as humic acid and tannic acid, which are commonly found in environmental samples. The master mix formulations use antibody-mediated hot-start chemistry to allow reaction setup at room temperature. Rapid hot-start activation and processive enzymes make them compatible with both standard and fast instrument cycling programs.
PCR or RT-PCR inhibitors, pipetting errors and thermalcycler malfunctions are common causes of inconsistent results. To control for these errors, we recommend including the Internal Positive Control (IPC) qPCR Inhibition Control Assay, which provides information on DNA polymerase performance, or the Internal Amplification Control (IAC) RT-qPCR Inhibition Control Assay, which provides information on reverse transcriptase and DNA polymerase performance. Both control assays are available separately.