Description
Easy Purification Workflow
Purify your DNA in five easy steps. The protocol requires only 30 minutes hands-on time. It has fewer processing steps than the competitor and doesn't require any chloroform.
Add CTAB, RNase, and Proteinase K.
Vortex.
Incubate for 30 minutes (difficult samples can be incubated for an additional 2 hours).
Centrifuge for 10 minutes.
Add 300μl of lysate and 300μl of Lysis Buffer into well 1 of the Maxwell® RSC cartridge—run time is 40 minutes.
Load the Maxwell® deck, add sample, and press start. It's as simple as that.
~100 minute protocol
Only 30 minutes hands-on time
Fewer processing steps
No chloroform
Add lysis buffer and proteinase K.
Incubate for 30 minutes at 60°C.
Centrifuge for 5 minutes.
Add chloroform to empty tubes.
Transfer supernatant from centrifuge step 4 and add to chloroform tubes.
Centrifuge for 15 minutes.
Add buffer 1 to a clean tube, add aqueous phase from step 8 and vortex.
Add solution to spin column.
Centrifuge for 1 minute.
Discard flow through and add buffer 2.
Centrifuge, discard flow through and centrifuge again.
Add buffer 3.
Incubate for 1 minute.
~120 minute protocol
90 minutes hands-on time
More processing steps
Requires chloroform
