Promega, CS3019A02, Lumit® Canine FcRn Binding Immunoassay

Unleash A Faster, Simpler Canine Immunoassay
Very little is known about the functional characteristics of canine IgG subclasses, including to what degree the various classes bind Fc receptors, engage the immune system or their half-life in serum. Understanding the binding affinities of the canine IgG subclasses to immune effector proteins such as Fc gamma receptors (FcγR) and neonatal Fc receptor (FcRn) is critical for gaining insight into canine immunology and for the discovery and development of therapeutic monoclonal antibodies for the treatment of canine malignancies. Given the nascency of, but rapid growth and interest in, the canine immune-oncology field, there is a substantial need for biologically relevant methods to characterize binding properties of canine immunoglobulins. The Lumit® Canine FcRn Immunoassay is a novel competition binding assay to aid researchers in evaluating canine IgG molecules. Lumit® assay technology is based on NanoBiT® luciferase complementation. LgBiT-labeled tracer binds to SmBiT-labeled cFcRn target, resulting in protein complementation and generation of bright luminescent signal. Addition of test antibodies that bind to cFcRn causes displacement of tracer, resulting in dose-dependent decrease in luminescence.