Promega, CS3271A08, FAO-Glo™ Fatty Acid Oxidation Assay

A Better Way to Measure FAO in Live Cells
Fatty acid oxidation, also known as beta-oxidation, is the metabolic process by which fatty acids are broken down in the mitochondria to generate energy. This process produces NADH and FADH 2 , which feed into the electron transport chain to drive ATP synthesis. FAO plays a critical role in cellular energy metabolism, especially during periods of fasting, exercise or metabolic stress, and is tightly linked to mitochondrial health and function. Understanding and measuring FAO is essential for studying metabolic diseases, obesity, diabetes and cancer.
Existing approaches to FAO measurement are either indirect, low throughput, or require specialized equipment. In contrast, the FAO-Glo™ Assay offers direct detection of FAO in a simple, plate-based format:
No radioactivity
No oxygen consumption proxies
No expensive instrumentation.
It combines true functional analysis with scalability and speed for modern metabolism research.