Promega, CS3631A05, Canine FcγRIII ADCC Bioassay

Fetch the Most Reliable Data with the Canine FcγRIII ADCC Bioassay
Canine Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) is an immune-response mechanism in which antibodies bind to a target cell, such as a virus-infected or cancerous cell, and recruit natural killer (NK) cells for their cytotoxic effector functions. There are several challenges to measuring the ADCC activity of canine immunotherapeutics including lack of standard protocols, limited species-specific assays and high levels of variability in primary effector cells.
The Canine FcγRIII ADCC Bioassay is a simple and robust system that can be used to measure the ADCC activity of canine antibody drugs that bind canine FcγRIII. The assay consists of a stable reporter cell line that reduces the variability and challenges of isolating canine PBMCs for primary cell assays.