Promega, CS3633B14, TLR7 and TLR8 Bioassays

Functional Detection of ssRNA and Small-Molecule Agonists
TLR7 and TLR8 are endosomal pattern-recognition receptors that recognize single-stranded RNA (ssRNA) and small-molecule agonists, playing a key role in antiviral immunity and innate immune activation. Bioassays targeting TLR7 and TLR8 provide mechanism-of-action-based platforms for the detection and quantitation of agonist activity in research, drug development and quality control settings. These assays enable precise functional readouts of receptor activation, offering biologically relevant data that cannot be captured by physicochemical methods alone.
Assay Utility and Format Advantages
Available in thaw-and-use reporter cell formats, TLR7 and TLR8 bioassays streamline immunostimulatory profiling by eliminating the need for primary cells or complex culture systems. Their sensitivity to ssRNA structure, sequence context and delivery formulations makes them ideal for screening adjuvants, characterizing innate immune modulators and assessing off-target immunogenicity in RNA-based therapeutics. These bioassays facilitate robust, reproducible assessments of TLR-mediated activity, supporting both early-stage discovery and lot-release testing.