Promega, E1330, pmirGLO Dual-Luciferase miRNA Target Expression Vector

Name: pmirGLO Dual-Luciferase miRNA Target Expression Vector
Brand: Promega
SKU: E1330

Evaluate microRNA Activity by Inserting miRNA Target Sites 3´ of the Firefly Luciferase Gene, Reporter activity correlates with miRNA activity, Optimized, luc2, reporter gene provides highest expression while the, Renilla, luciferase gene provides normalization, The moderate-strength PGK promoter provides more biologically relevant analysis not possible with strong promoters, Configuration: 20μg

Catalog Number: E1330

Pricing

Send an RFQ.

View RFQ List

Category

Expression Vectors

TagsLuciferase Assays Genetic Reporter Vectors and Cell Lines

Description

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene ( luc2 ). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase ( hRluc-neo ) acting as a control reporter for normalization using the Dual-Luciferase® Assay and selection for stable cell lines.
Applications
miRNA detection.
miRNA binding site analysis.
Transcript stability study.
3´-UTR analysis.
Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.
Sequence Information
pmirGLO Vector GenBank® Accession Number FJ376737 and vector sequence text file .
Mechanism of action of the pmirGLO Vector.

Specifications

General

Catalog Number	E1330

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.