Promega, E2312, FuGENE® HD Transfection Reagent

Unrivaled Transfection Performance
Transfection of Stem Cells
“FuGENE® HD resulted in the highest stable transfection rate, estimated by antibiotic selection, with plasmids containing genes under the control of the EF1alpha or PGK promoters”.
Liu J., et al. (2009) Stable transgene expression in human embryonic stem cells after simple chemical transfection. Mol. Reprod. Dev. (2009) 76, 580-6.
Transfection of Cancer Cell Models
FuGENE® HD Transfection Reagent lets you work in your cancer model. Use the FuGENE® HD protocol database to see if transfection conditions for your cell line are available. The database includes over 25 cancer models and provides transfection protocols specific for each cell line.
Cellular Imaging
High expression levels and the low toxicity of FuGENE® HD can bring better performance for imaging experiments. Read more about the low toxicity of FuGENE® HD in:
Antczuk et al. (2014) A High Content Assay to Assess Cellular Fitness. Comb Chem High Throughput Screen . 17(1), 12–24.
Protein Production
FuGENE® HD is made without animal products and is compatible with defined media. This means you can conduct experiments in mammalian and eukaryotic cells with confidence.