Promega, E2940, Dual-Glo® Luciferase Assay System

Name: Dual-Glo® Luciferase Assay System
Brand: Promega
SKU: E2940

Detects firefly and, Renilla luciferase internal control provides more accurate results, Assay cells directly in growth medium for both reporters, Extended half-life of 2 hours for each reporter, Configuration: 100ml

Catalog Number: E2940

Pricing

Send an RFQ.

View RFQ List

Category

Media Bottles

Pack Size1 vial

TagsLuciferase Assays Reporter Assays

Description

An Extended-Life Dual-Luciferase Assay with "Add-Mix-Measure" Convenience
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.
With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.
The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications.
Assay Advantages
Increased Precision and Accuracy. Assay normalization using an internal control co-reporter minimizes the effects of variations in cell number and health, transfection efficiency and nonspecific cellular responses.
No centrifugation or pre-lysis steps required. Reagents are added directly to growth medium for both reporters.
Stable signal allows flexibility for batch or continuous processing of 96- and 384-well plates. Each luminescent signal can be measured for up to 2 hours after reagent addition.
Simple, two-step assay is compatible with any luminometer. On-board injectors not required.
Wide Dynamic Range allows analysis of high and low reporter activity without sample dilution. Linear over at least 6 logs of enzyme concentration for each reporter.
Learn more about custom options for this product at: www.promega.com/custom/ Contact Promega for information on bulk purchases.

Specifications

{"type": "table", "rows": [{"name": "Dual-Glo® Luciferase Substrate", "conditions": {"Part #": "E297B", "Size": "1 × 1 vial"}}, {"name": "Dual-Glo® Luciferase Buffer", "conditions": {"Part #": "E298B", "Size": "1 × 100ml"}}, {"name": "Dual-Glo® Stop & Glo® Substrate", "conditions": {"Part #": "E313B", "Size": "1 × 1,000μl"}}, {"name": "Dual-Glo® Stop & Glo® Buffer", "conditions": {"Part #": "E314B", "Size": "1 × 100ml"}}]}

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.