Description
Improved Sensitivity vs. Absorbance and Other Fluorescent ssDNA Quantitation Systems
For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased compared to absorbance at 260nm (NanoDrop® method) and Qubit® ssDNA.
The dynamic range of the assay is wider than that of Qubit® ssDNA and equivalent to OliGreen® reagent.
*Based on 1µl sample input per assay. Quantitation of more dilute samples is possible using more input dsDNA per assay.
ssDNA Quantitation Sensitivity and Assay Linearity
The QuantiFluor® ssDNA System detects as little as 1ng/ml ssDNA in a 96-well microplate assay (corresponding to 1µl of a 200ng/ml starting sample). Detection limit is defined as greater than three standard deviations above background RFU. Sensitivity is equivalent to Quanti-IT™ OliGreen® reagent.
Easy Setup on Microplate or Single-Tube Fluorometers
The QuantiFluor® ssDNA System includes all the necessary reagents to quickly set up and quantitate single-stranded DNA, and is easy to set up on microplate or single-tube fluorometers. The System is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. Compatible Promega fluorometers include:
(Use Blue Channel Ex 460nm, Em 515–575nm)
GloMax® Discover
GloMax® Explorer
How Do Fluorescent Dyes Compare to Other Quantitation Methods?
In these short videos, Promega Applications Scientist Doug Wiezorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
