Promega, E4981, ViaFect™ Transfection Reagent

Create Assays Not Possible With Other Reagents
Assay for cytokine signaling in a hematopoietic cell model.
TF-1 suspension cells were transiently transfected with pGL4.32[luc2P/NF-κB-RE/Hygro] Vector, an NF- κB response element luciferase reporter, using either ViaFect™ Transfection Reagent at a 2:1 reagent:DNA ratio or Amaxa Nucleofector® II (electroporation). The following day cells were stimulated with TNFα for 6 hours, and the response was measured with Bio-Glo™ Luciferase Reagent.
Directly assay HIF1α protein stability in iPS-derived cardiomyocytes.
iCell® Cardiomyocytes (Cellular Dynamics) were transfected with ViaFect™ Transfection Reagent using pNLF1-HIF1A [CMV/neo] Vector, a HIF1A-NanoLuc® Luciferase fusion reporter, at a 4:1 reagent:DNA ratio. Twenty-four hours post-transfection cells were treated as indicated with hypoxia mimetics for 3 hours, and the amount of HIF1α-NanoLuc® protein was detected using Nano-Glo™ Luciferase Assay Reagent.
Assay for hypoxia transcriptional response in iPS-derived cardiomyocytes.
iCell® Cardiomyocytes (Cellular Dynamics) were transfected with ViaFect™ Transfection Reagent using the pGL4.42[luc2P/HRE/Hygro] Vector, a hypoxia response element luciferase reporter, at a 4:1 reagent:DNA ratio. Twenty-four hours post-transfection, cells were treated with hypoxia mimetics as indicated for 6 hours followed by luciferase detection using ONE-Glo™ Luciferase Assay System.