Description
Design Features
Frequently Asked Questions
The Quantus™ Fluorometer is an easy-to-use and affordable single-tube fluorometer. The compact instrument offers intuitive navigation and a large 3-inch liquid crystal display screen. The fluorometer is preprogrammed with protocols for single-stranded DNA, double-stranded DNA and RNA quantitation using the QuantiFluor® Dyes but is also compatible with other fluorescent assays.
The Quantus™ Fluorometer is equipped with two fluorescence channels for nucleic acid and protein quantitation: Blue fluorescence channel: Excitation 495nm shortpass (wavelengths up to 495nm), emission 510–580nm. Red fluorescence channel: Excitation 640nm shortpass (wavelengths up to 640nm), emission 660–720nm.
When used with the QuantiFluor® Dyes, the Quantus™ Fluorometer provides sensitivities that are up to 40,000 times higher than that of absorbance-based quantitation, depending on the assay used. As little as 50pg/ml dsDNA can be detected using the standard QuantiFluor® dsDNA Dye protocol. Custom protocols can be created to detect even lower amounts (as little as 1pg/ml dsDNA). The instrument has a linear range that encompasses five orders of magnitude.
No. The Quantus™ Fluorometer is designed to operate as a standalone instrument. The Quantus™ Fluorometer displays fluorescence measurements in real time but also saves and displays the 50 most recent measurements in the results history. However, the fluorometer can be connected to a computer to transfer data in real time after installing the free Quantus™ Software. To download the software, visit the Quantus™ Software download page.
To download and use the Quantus™ Software, your computer must have the Windows®10, Windows® 8 or Windows® 7 (32- or 64-bit) operating system, a .NET Framework 4.0 or later and at least one USB port available.
The Quantus™ Fluorometer accepts 0.5ml PCR tubes. We recommend thin-walled PCR tubes (Axygen thin-walled PCR tubes, Axygen Cat.# PCR-05-C, available from Fisher or VWR) for the best optical clarity.
The fluorometer requires a power source of 5V, 0.2A maximum. The Quantus™ Fluorometer is supplied with a USB Cord to plug the instrument in to a computer and a USB Power Supply and Outlet Adapter Kit, which includes four adapters, to plug the instrument in to the wall outlet.
To calibrate, simply measure the fluorescence of your blank sample and the standard that represents the highest point on your standard curve. The Quantus™ Fluorometer saves the calibration data for future measurements. The fluorometer must be calibrated prior to the first use of a protocol. If a protocol that has never been calibrated is selected, the Calibration screen will appear automatically. Once the protocol of interest is calibrated, the Quantus™ Fluorometer will save the calibration values for future use and will not require recalibration. However, we recommend performing a calibration with each new lot of dye reagent to ensure the most accurate measurement.
Yes. The Quantus™ Fluorometer comes with a 1-year warranty upon purchase of the instrument. Warranty coverage includes all parts, labor and shipping to and from the repair location.
QuantiFluor is a registered trademark of Promega Corporation. Quantus is a trademark of Promega Corporation. Windows is a registered trademark of Microsoft Corporation.
