Promega, E6495, ViviRen™ Live Cell Substrate

Name: ViviRen™ Live Cell Substrate
Brand: Promega
SKU: E6495

Sensitive Quantitation of, Engineered coelenterazine for live-cell reporter gene, BRET and RNAi assays, Permits real-time measurement in multiwell plates, High signal-to-background ratios, Configuration: 37mg

Catalog Number: E6495

Pricing

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Category

Cell Imaging Plate Readers

TagsLuciferase Assays Reporter Assays

Description

Generate Kinetic Profiles for Reporter Assays, BRET and RNAi in Living Cells
ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity.
Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are each supplied as a lyophilized solid.
Assay Advantages
Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers.
Unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine.
Multiplex with CellTiter-Glo® and other lytic assays for improved accuracy and precision.
Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition. CHO cells stably expressing Renilla luciferase were exposed to 20µM ViviRen™ Live Cell Substrate or coelenterazine approximately 1.5 minutes after continuous luminescent reading was initiated. For more details, see Figure 5 of TM064.

Specifications

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Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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